Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precisionAnalytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Validate the optical performance of your absorbance . Cells were transfected with Sirt1 siRNA or scrambled siRNA. The instrument uses a paired monochromator and xenon flash lamp for absorbance reads allowing for optimization of wavelengths between 200 nm and 1000 nm. spr * SpectraMax i3(x) SpectraTest ABS2 v1. S. 2. For more detailed information and technical support of Molecular Devices instruments or software,The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. Molecular Devices SpectraMax M3 Laboratory Equipment PDF Operation & User’s Manual (Updated: Wednesday 18th of October 2023 10:44:56 AM) Rating: 4. 7 mm for all shake intensities. I used the same plate and set the PMT to be automatic. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. A single colony of bacterial isolates was inoculated in Marine Broth using a sterile inoculation loop. Page 12 SpectraMax MiniMax Imaging Cytometer Installation Guide 10. . The bacterial colony was mixed with. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. SoftMaxProSoftwareUserGuide 2 5014177G ThisdocumentisprovidedtocustomerswhohavepurchasedMolecularDevices,LLC(“Molecular Devices”)equipment,software,reagents. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. It does not work as expected. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. Then, 25 nM HOPS complex, 50 nM Sec18, 600 nM Sec17, and finally 100 nM Vam7 were added. 7. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. 2. Molecular Devices’ unique SpectraDrop™ MicroVolume Microplate offers the highest throughput solution for low volume measurement available on the market today. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Stray light. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. 発光モードに対応した機種（SpectraMax M5e/M5/M4/M3）では、発光モード専用のフォトンカウント検出器も搭載しました。 蛍光に特化した検出器を流用するのではなく、専用の光学系を採用することで、発光、TR. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. 2. 1) Select the correct plate and the wells that you want to measure. . Description 1. The SpectraMax Multi-Mode Microplate Reader offers both the Cuvette. Our luminescence plate readers, also referred to as luminometers are ideal for measuring a wide range of assays including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminescence. Our SpectraMax microplate readers and SoftMax Pro software are the industry's most cited and have empowered life science researchers to advance protein and cell biology―breaking the barriers to novel, landmark discoveries. Molecular Devices’ unique SpectraDrop™ MicroVolume Microplate offers the highest throughput solution for low volume measurement available on the market today. The optical density was read using a SpectraMax M3 (Molecular Devices, Sunnyvale, CA, USA). Light Source: Xenon Flash Lamp (1 joule/flash) Detectors: 2 photomultiplier tubes. o For example: Ex: 485/Em 525/Cutoff 515 4. 2. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. $ 15,999. The M4 Reader offers the same modes as the M3 Reader, plus time-resolved fluorescence,. For more detailed information and technical support of Molecular Devices instruments or software,The SpectraMax® iD3 Multi Mode Microplate . By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Plate Formats: 6, 12, 24, 48, 96, 384 wells. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well Luminescence is the emission of light by a substance as a result of a chemical reaction called chemiluminescence or an enzymatic reaction called bioluminescence. SoftMax Pro GxP is a special software release that includes full user and permission con-SpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader TRF,HTRF,FP HTRF, FP HTRF N/A Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. , 2020). Recommended SpectraMax iD3/iD5 reader settings. 次いで、ユーザーのラボにとって最も役立つ機能を有するモデルを自由に. Figure 1. 1. Operated fromTo ensure loading of equal amounts of protein for each sample, colorimetric protein quantification was performed using a Bio-Rad Protein Assay (Bio-Rad, Hercules, CA) and a SpectraMax M3 spectrophotometer (Molecular Devices, Sunnyvale, CA). 1. Read mode. Then, the absorbance was measured using a spectrophotometer (Spectramax M3, Thermo Scientific, Waltham, MA, USA) at 734 nm. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. SpectraMax Luminometer Luminescence detection typically offers greater sensitivity than absorbance or fluorescence detection. SpectraMax i3 Multi-Mode Platform A flexible, user-upgradeable microplate detection system Key Features The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. After cooling to room temperature (22 °C), the mixture was transferred to 96-well plates. The innovative and flexible design features. 100 µL of MycoAlert Substrate (reconstituted in MycoAlert Buffer) was immediately added to each well and the plate incubated at room temperature for 10 minutes. 115 SpectraMax M4 Multi-Mode Microplate Reader. STAKMAX. Category 2 and 3 microplate readers use gradient heating as well. The following document is intended to demonstrate setup of this instrument. Dual monochromatic, three modes detection, UV Vis Abs, Fluor, and Lum, cuvette. We would like to show you a description here but the site won’t allow us. FI assay. Total phenolic contents were calculated from the gallic acid standard curve. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. The integration time suggested is 5-10 sec. 312 gain calibration failed. Expand your lab’s capabilities to include flash applications such as dual luciferase and. For more detailed information and technical support of Life Technologies assays including specific conditions for assay windows between 2-3 fold, please call 1-800-955-6288 and enter extension 40266 or email. G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax i3x, SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 Linearity G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax. Cables. SpectraMaxM3,M4,M5,andM5eMicroplateReaderUserGuide 50 0112-0115G Item Description Wavelengthrange 200-1000nm Wavelengthselection Monochromator,tunablein1nmincrementsPage 25 SpectraMax iD3 Multi-Mode Microplate Reader Installation Guide To install the transport lock on the plate drawer: 1. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. spr SpectraMax iD5 SpectraTest ABS2 v1. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME. gingivalis -treated BMDMs (MOI = 50), with or without pretreatment of OTTSP167 or Compound 50 for 2 h. Description 1. com. Also, when using a SpectraMax M4 Reader, the GxP audit trail incorrectly re ported the instrument as a SpectraMax M3 Reader. We would like to show you a description here but the site won’t allow us. 0112-0112F 3 Contents Chapter1:SpectraTestABS1ValidationPlateOverview 5 CertificateofCalibration 6 ISO-17025 6 AvailableTests 7 Chapter2:ValidationProtocols 9SpectraMax® L (software version 5. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part. 2. Using a SpectraMax M3 spectrophotometer from Molecular Devices, we measured the color change at 450 nm. It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. Click template editor icon in Home panel or select configure your plate layout in plate setup helper a. 1 release preventing the protocols from being used for validation. In the SoftMax Pro Software, select the Operation tab and click Change Aperture to display a message. However, limitations such as engraftment failure and time-consuming for establishing and expanding PDX models followed by testing drug efficacy, and inability to subject to. Fluorescence was measured using a SpectraMax M3 plate reader (excit/emiss = 480/520 nm). The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. 90-0083 series): Excitation 530 nm,. SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. We would like to show you a description here but the site won’t allow us. Plate chamber design of the SpectraMax category 1 microplate readers. Also refer to this Multimode Reader Comparison Chart. Also refer to this Multimode Reader Comparison Chart. reader in the visible range between 405–750 nm with NIST and NMI. The SpectraMax ABS Plus reader utilizes Molecular . The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Generate more data points without the need to dilute. 2. For performing enzyme assays SpectraMax M3 Multi-Mode Microplate Reader (Molecular device, USA) was used. Mitochondrial activity was expressed by the decrease in OD (450–660 nm), discounting the average of the ODs of the controls . 1. BioTek Synergy LX Multi-Mode Microplate Reader (FLD/Lumi) Accuris MR9600 SmartReader 96 and MW9600 Smartwasher 96 Bundle, 115V. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. UV Qualification, using NIST traceable standards, is now available for SpectraMax 190, Plus 384 and the M Series (M2/M3/M4/M5/M5e) Parameters that are checked during the UV Qualification are: Wavelength accuracy. Bithionol (2 μg/ml) and vancomycin (4 μg/ml) were used as controls. Firefly luciferase, a 61 kD monomeric protein, is especially attractive to many researchers because of its high sensitivity, wide linear detection range and extremely low background due to the absence of endogenous luminescent activity in mammalian cells. Our Price: $8,635. The result was expressed as mg gallic acid. , Sunnyvale, CA). Cables. Find many great new & used options and get the best deals for Replacement Bulb for Molecular Devices SpectraMax M3 Flash Lamp at the best online prices at eBay! Free shipping for many products! Skip to main content. Sample Fluorescent Reader Setting for the ADP Hunter™ Plus (Cat. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. We would like to show you a description here but the site won’t allow us. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. 더 많은. Microplate Readers Molecular Devices SpectraMax M2E Microplate Reader. 314 low lamp level warning. Metrics used to measure sensitivity include signal-to-background ratio (S/B), signal-to-noise ratio (S/N), lower limit of detection (LLD) and Z’-factor 1. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode Detection Platform. We would like to show you a description here but the site won’t allow us. 00 . Note: you might need to slide the microplate reader on the foam pad to make room for the cytometer. Wavelength precision. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. . . Filterless Absorbance Microplate Readers provides fast & consistent results with steady temperature enabling a wide range of assays - ELISA reader, microbial growth, & protein quantitation. Plant material. 3. Dual monochromators increase performance and assay flexibility. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Data were analyzed using Parallel Line Analysis 2. SpectraMax Gemini, SpectraMax I series, SpectraMax M series, FlexStation 3. Our SpectraMax microplate readers and SoftMax Pro software are the industry's most cited and have empowered life science researchers to advance protein and cell. SpectraMax Microplate Readers Multi-mode Readers Single-mode readers SoftMax Pro Software GxP Compliance Solutions SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Cell Degranulation. 2. Mitosox works fine in 96 wells. Italic and boldface type are used for emphasis. 72 MB. ZERO BIAS - scores, article reviews, protocol conditions and moreF1 SpectraMax Plus384andABSPlus: Certificate Value – 2≤ Average Peak Value≤ Certificate Value + 2 SpectraMax M2,M2e,M3,M4,M5,andM5e: Certificate Value – 3≤ Average Peak Value≤ Certificate Value + 3 Photometric Accuracy F2, F3, F4 AverageOD=Certificate Value± (Certificate Value(0. 0 mm hex key to remove the six blue retaining screws that secure the transport bracket (B) to the instrument. Cells cultured in 96 well plate are washed with PBS or HBSS (phenol red free), incubated with ~5-10 uM. Application note demonstrating that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. Aggregation of MDH was monitored as a function of light scattering at 360 nm over 30 min at 51°C in a SpectraMax M3 (Molecular Devices, United States) microplate spectrometer. The SpectraMax M2 and M2e are versatile and reliable instruments that can perform a range of applications in. SpectraMax M3, M4, M5, and M5 e. Use the 3. Bioz Stars score: 86/100, based on 1 PubMed citations. Instructions for using SIRT1 siRNA and scrambled siRNA were provided by Santa Cruz Biotechnology (CA, USA). 1) EarlyTox Glutathione Assay (6. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. "With this complete portfolio of multi-mode configurations, we can deliver multi-mode. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. Tailored to your different needs, these kits are configured and optimized for Molecular Devices SpectraMax® microplate readers with preconfigured protocols provid-すべてのSpectraMax® Mシリーズマルチモードマイクロプレートリーダーで、マイクロプレートおよびキュベットを使用して、紫外光・可視光吸光度ならびに蛍光強度を測定できます。. 4. 304 signal level saturation. . a SpectraMax M3 microplate reader (Molecular Devices Inc. Preconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. FlexStation™ III. Microplate and read height should be optimized for each new lot of microplates or assay volume used (use the Show Pre-Read Optimization Options in the instrument settings in SoftMax Pro Software. The luminescent signal was proportional to the amount of GSH or GSSG present. Case Study 1 — Molecules Showed High Conformational Similarity: In the first case study, we analyzed multiple batches of innovator Herceptin (trastuzumab) and biosimilar MAbs using the antibody-array ELISA. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. Filterless readers provide consistent results with steady temperature regulation. SpectraMax M3 Plate Read, Plate Map, Data Analysis and Export Setup pg. Description 1. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Features. Then aluminum. D 1 1. This process should take about 3 - 5 minutes. Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precision Analytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). With optimized reagents, option to operate the. Fluorescence of the samples were read using a SpectraMax M3 instrument with an excitation set to 355 nm and emission set to 460 nm. 4/5 Set up plate template / Define Plate Map 1. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. Tecan Infinite 200 PRO M Plex Multimode Microplate Reader 30050303. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. For SpectraMax L or LMax II, LMax II. Call 516-939-0888 to check current. 0 mm hex key. ISM Equipment Classification (Group 1, Class A) This equipment is. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. book Page 1 Wednesday, July 7, 2010 2:09 PM. The drawing shows a standard microplate of 15 mm height covered with a lid. All associated StakMax® stackers. Finally, the absorbance was measured with a SpectraMax M3 microplate reader (Molecular Devices Corporation, Sunnyvale, CA, USA) at a wavelength of 450 nm. iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. spr * SpectraMax i3(x) SpectraTest ABS2 v1. Fluorescence Microplate Reader Comparison Chart. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. Fluorescence was quantified on SpectraMax M3 plate reader, Ex 485/Em 520 using SoftMax Pro software. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. 1). After lysis of the cells, the mixture of 30 µL cell supernatant and 150 µL 30 µg/mL DTNB incubated for 25 min. The actual pathlength, d, of a solvent is found from the following equation: d cm. Acarbose was used as a. The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. In addition, they allow to increase their performance sequentially from models: M2 ‘ M2e;. 0200-7027. As for each measurement, I. SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3Chapter 1: Introduction The SpectraMax® Mini Multi-Mode Microplate Reader is a multi-detection instrument with a 6- well to 384-well plate read capability. The following document is intended to demonstrate setup of this instrument. 4. The ProcartaPlex 384-well format was specifically developed to increase sample throughput while maintaining the robustness of a 96-well assay. Fluorescence (FL). S. When the user applies a set temperature of 37°C, the top is heated to 37. Accurate quantitation The optical design of the SpectraMax 340PC384 reader truly mimics a dual. 0 mm hex key to tighten screws #2 and #3 until the lock is attached to the plate drawer. Add to Cart. Devices PathCheck® Sensor—the only patented technology available that measures the depth (optical pathlength) of samples in a microplate. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. The resorufin product formed was monitored by fluorescence spectroscopy (excitation energy at 530 nm and emission energy at 590 nm) every 10 min in Spectramax M3 (Molecular Devices). 4. 190-1000 nm. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 2. Add to Cart. Lift the right side of the microplate reader and place the feet into the two mounting cups on the baseplate. Align the two slots on the bottle holder with the two pegs on the rail. A colorimetric assay was performed using a SpectraMax M3 microplate reader (Molecular Devices, Sunnyvale, CA) at 450 nm. Page 28 Chapter 5: Read Modes and Read Types 1. 313 reference gain check fail. U2OS cell viability (%) = [A cells with different treatments −A contro ]/[A cells with no treatments − A control ] × 100. Transcreener ® ADP. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. no. 1. item 6 White 18-SMD LED License Plate Lights For 04-06 BMW E46 LCI 325Ci 330Ci M3 Coupe White 18-SMD LED License. ATP standard curves read on SpectraMax microplate readers. SpectraMax Spectrofluorometers. The DNA extracts were quantified using Quanti-iT™ Picogreen ® dsDNA reagent (Invitrogen, Paisley UK) on a black 96 well plate at an absorbance of 520 nm. Amplitude : 1. 1. Microplate Readers include the M3, M4, exception is noted. Offer Ends In: 5 Days 05: 02: 03. (2005) . The absorbance was assessed at 405 nm by a SpectraMax M3 microplate reader (Molecular Devices, Sunnyvale, CA, USA). 5065390D 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 ComputerIntegration 8 PlateControls 9 CuvetteChamber 10The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). SpectraMax M3 Multi-Mode Microplate Reader. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellThe SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro Data Acquisition and Analysis Software. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. All configurations offer a triple-mode cuvette port, accurate temperature control, microplate shaking and comprehensive dataPlates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. Microplate Readers Molecular Devices Spectramax iD3 Microplate Reader. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quantitation of firefly luciferase expression in mammalian cells. Operated from within SoftMax Pro Software, the StakMax Microplate Handler can hold up to 50 plates and facilitates barcode reading. . The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. Absorbance was read at 570 nm using a SpectraMax M3. NETosis Assays Using Immunostaining and Microscopy. 10. The SpectraMax M3 Microplate Reader is supplied with SoftMax Pro Data Acquisition and Analysis Software, Molecular Devices' industry leading all-in-one software. 3: The cuvette chamber. Multimode Reader Comparison Chart. For more sample throughput, the system canStakMax Handling Stacker Base Unit. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. Examples: “Press carefully to engage,” “ Do not. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Absorbance was measured at 490 nm on a Molecular Devices SpectraMax M3 plate reader with SoftMax Pro 6. The corrected absorbance is within 5% of the. SpectraMax® Paradigm® Multi-Mode Detection Platform. To determine growth rate, the log phase of growth. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. ZERO BIAS - scores, article reviews, protocol conditions and moreInstruments and Settings for Fluorescence Read-out (Other Fluorescent Assays) FLUOstar Omega, VANTAstar, CLARIOstar, PHERAstar, PolarStar Optima. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. es • Flexibility for your assay needs: Robotics compatibility for increased throughput:The bound CV was dissolved in 30% acetic acid and the absorption was measured in a microplate reader at OD 590 using SpectraMax M3 (Molecular Devices, United States). Microplate Readers include the M3, M4,. book Page 1 Wednesday, July 7, 2010 2:09 PMThe fluorescent intensity was measured using SpectraMax M3 fluorescence microplate reader (Molecular Devices) every 90 seconds with excitation at 320 nm and emission at 420 nm at 37°C for 2 hours. Keywords: Spectramax M2 Systems, Spectramax M2e Systems, Spectramax M2/m2e Readers, Pathcheck Sensor, Spectratest Abs1 Absor Jun 8th, 2023SpectraMax M3, M4,. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. 1step assay with SpectraMax microplate readers. Molecular Devices VersaMax Microplate Reader. Description 1. SpectraMax M5/M5e/M4/M3/M2/M2e. Published Dec 16, 2020 | Updated Dec 16, 2020. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. To avoid injury, it is recommended that two. The Molecular Devices® SpectraMax® M3 Reader provides three detection modes: UV-Vis absorbance, fluorescence intensity, and luminescence, but can be upgraded to include time-resolved fluorescence and/or fluorescence polarization modes. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email [email protected] 21 Chapter 2: Set Up the StakMax Microplate Handler 4. WARNING! The instrument weighs approximately 36 pounds (16. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. SpectraMax M2, M2e;. At each time point, cells were incubated with CCK-8 reagent for 1 hour at 37 °C, followed by measuring the optical absorbance at 450 nm with the SpectraMax M3 microplate reader (Molecular Devices. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. Examples: “Press carefully to engage,” “ Do not press down. 0 out of 5 $ 12,499. For example, microplates containing different samp les can be read using the same orCell viability는 세포막 Integrity 또는. Immunofluorescence ASC antibody (1:200, Abcam, ab155970, UK) was used for immunofluorescence in P. SpectraMax® i3 Multi-ModeDetectionPlatform withtheSpectraMax® MiniMax™ImagingCytometer UserGuide 5025027A April2013The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The SpectraMax M3 microplate reader (Molecular Devices, USA) was used to monitor the colour development over 5-min intervals for 30 min at 370 nm. no. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was. The SpectraMax i3 Multi-Mode Plate Reader enables characterization of proteins by measuring intrinsic tryptophan fluorescence. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. D 1 1. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Slide the tip into the nozzle, the slots are labeled 1 and 2 for injector 1 and injector 2. For all. 315 can't find zero order. When the user applies a set temperature of 37°C, the top is heated to 37. All the experiments were done in triplicate. Learn how to set up protocols, customize reports, export results, and more with this PDF document. 305 reference level saturation. Data of selected analytes in the graphs below illustrate sample correlations between 96-well and 384-well format of 16 human plasma samples. book Page 1 Tuesday,. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. ATP concentration (M) Average RLU SpectraMax L SpectraMax i3x (cartridge) SpectraMax Mini SpectraMax iD5 SpectraMax i3x (onboard) SpectraMax M5eThe SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. Choose from a three- (M3), four- (M4), orThe SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. All procedures completely complied with the manufacturer’s instructions. We would like to show you a description here but the site won’t allow us. HNE (EC 3. 0 out of 5Growth curves were read in a Spectramax M3 plate reader, at 37 °C with shaking at an optical density of 600 nm; reads were taken every 5 min. 2 only) SpectraMax M-series Microplate readers. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. AquaMax® 4000. com. If no sound is heard during initialization, contact technical support. SpectraMax Mini. edu, 814-863-3503.